No recognition of MHC class II+ cells infected with a vaccinia virus encoding influenza type A nucleoprotein by class II-restricted T cells
Identifieur interne : 001E72 ( Main/Exploration ); précédent : 001E71; suivant : 001E73No recognition of MHC class II+ cells infected with a vaccinia virus encoding influenza type A nucleoprotein by class II-restricted T cells
Auteurs : Giulia Freer [Italie] ; Sonia Senesi [Italie]Source :
- Immunology Letters [ 0165-2478 ] ; 1993.
English descriptors
- Teeft :
- Assay, Braciale, Charge class, Clone, Cytolytic, Cytolytic activity, Cytoplasmic, Encoding, Endogenous cytoplasmic protein, Exogenous growth factor, Final concentration, Growth factor, Human system, Immunol, Influenza, Influenza nucleoprotein, Karyophilic sequence, Kind gift, Lysed, Matrix protein, Mouse cells, Nuclear protein, Open bars, Peptide, Peptide transporter, Proliferation assays, Release assay, Release assays, Same cells, Solid bars, Splenocytes, Spontaneous release, Such cells, Synthetic peptides, Target cells, Vaccinia, Vaccinia virus, Vaccinia virus encoding, Viral infection, Viral proteins.
Abstract
Abstract: MHC class II is mostly charged by antigens derived from the outside of the antigen-presenting cell (APC), while class I presents endogenous antigens transported as peptides to the endoplasmic reticulum (ER) by specific transporters. Nevertheless, many antigens, especially glycoproteins, can be presented in vitro by class II even if endogenous. In order to investigate the class-II-restricted T-cell response to endogenously synthesized influenza nucleoprotein (NP) synthesized in infected cells as a model of non-glycosylated nuclear protein, class-II-restricted cytolytic T-cell (CTL) clones were established from BALB/c (H-2d) mice immunized with either influenza A/PR/8/34 (PR8) strain or with a vaccinia virus encoding the NP protein. Two of the clones were characterized in detail and turned out to be cytolytic, I-Ad-restricted and NP peptide 218–229 specific. Even though an in vivo class-II-restricted T-cell response was elicited in BALB/c mice immunized with a vaccinia virus encoding nucleoprotein (Vacc-NP), class II+ mouse lymphoma cells were not lysed by the class-II-restricted clones in vitro when they were infected with the same virus or with a vaccinia virus encoding a truncated form of NP with no karyophilic sequence, showing that the de novo synthesized protein targeted to the nucleus or remaining in the cytoplasm cannot charge class II through the same pathway as class I in murine APC. These results extend previous observations made on transfected cells to cells that express an antigen during viral infection.
Url:
DOI: 10.1016/0165-2478(93)90105-B
Affiliations:
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Zeno 39, 56100 Pisa</wicri:regionArea><placeName><settlement type="city">Pise</settlement><region nuts="2">Toscane</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunology Letters</title><title level="j" type="abbrev">IMLET</title><idno type="ISSN">0165-2478</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="305">305</biblScope><biblScope unit="page" to="312">312</biblScope></imprint><idno type="ISSN">0165-2478</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-2478</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Braciale</term><term>Charge class</term><term>Clone</term><term>Cytolytic</term><term>Cytolytic activity</term><term>Cytoplasmic</term><term>Encoding</term><term>Endogenous cytoplasmic protein</term><term>Exogenous growth factor</term><term>Final concentration</term><term>Growth factor</term><term>Human system</term><term>Immunol</term><term>Influenza</term><term>Influenza nucleoprotein</term><term>Karyophilic sequence</term><term>Kind gift</term><term>Lysed</term><term>Matrix protein</term><term>Mouse cells</term><term>Nuclear protein</term><term>Open bars</term><term>Peptide</term><term>Peptide transporter</term><term>Proliferation assays</term><term>Release assay</term><term>Release assays</term><term>Same cells</term><term>Solid bars</term><term>Splenocytes</term><term>Spontaneous release</term><term>Such cells</term><term>Synthetic peptides</term><term>Target cells</term><term>Vaccinia</term><term>Vaccinia virus</term><term>Vaccinia virus encoding</term><term>Viral infection</term><term>Viral proteins</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: MHC class II is mostly charged by antigens derived from the outside of the antigen-presenting cell (APC), while class I presents endogenous antigens transported as peptides to the endoplasmic reticulum (ER) by specific transporters. Nevertheless, many antigens, especially glycoproteins, can be presented in vitro by class II even if endogenous. In order to investigate the class-II-restricted T-cell response to endogenously synthesized influenza nucleoprotein (NP) synthesized in infected cells as a model of non-glycosylated nuclear protein, class-II-restricted cytolytic T-cell (CTL) clones were established from BALB/c (H-2d) mice immunized with either influenza A/PR/8/34 (PR8) strain or with a vaccinia virus encoding the NP protein. Two of the clones were characterized in detail and turned out to be cytolytic, I-Ad-restricted and NP peptide 218–229 specific. Even though an in vivo class-II-restricted T-cell response was elicited in BALB/c mice immunized with a vaccinia virus encoding nucleoprotein (Vacc-NP), class II+ mouse lymphoma cells were not lysed by the class-II-restricted clones in vitro when they were infected with the same virus or with a vaccinia virus encoding a truncated form of NP with no karyophilic sequence, showing that the de novo synthesized protein targeted to the nucleus or remaining in the cytoplasm cannot charge class II through the same pathway as class I in murine APC. These results extend previous observations made on transfected cells to cells that express an antigen during viral infection.</div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Toscane</li></region><settlement><li>Pise</li></settlement></list><tree><country name="Italie"><region name="Toscane"><name sortKey="Freer, Giulia" sort="Freer, Giulia" uniqKey="Freer G" first="Giulia" last="Freer">Giulia Freer</name></region><name sortKey="Senesi, Sonia" sort="Senesi, Sonia" uniqKey="Senesi S" first="Sonia" last="Senesi">Sonia Senesi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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